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KMID : 0358320070480101044
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Korean Journal of Urology
2007 Volume.48 No. 10 p.1044 ~ p.1049
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The Effect of Muscarinic Receptor Subtype Antagonists on Detrusor Overactivity Induced by Bladder Outlet Obstruction in Rats
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Kim Su-Jin
Hwang Tae-Kon
Kim Joon-Chul
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Abstract
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Purpose:We studied the role of the muscarinic receptor subtype on the urothelium and detrusor activity in rats with detrusor overactivity induced by bladder outlet obstruction(BOO).
Materials and methods: Forty Sprague-Dawley rats were used for this study. They were divided into 15 controls and 25 experimental rats. Partial BOO was induced for 3 weeks and a sham operation was performed on the control group animals. A cystometrogram(CMG) was performed in 20 BOO and 10 control rats. During the CMG, M2 selective muscarinic receptor antagonist(methoctramine) and nonselective muscarinic receptor antagonist(tolterodine) drugs were administrated intravesically. The contraction intervals and pressure were evaluated. Bladder muscle strips were prepared from five BOO and five control rats. The contractile responses were evaluated at 2-, 4-, 8-, 16-, and 32Hz field stimulation in the control and BOO groups and after administration of the methoctramine and tolterodine.
Results:The results of the CMG showed that the rats in the BOO group had a decreased contraction interval and increased contraction pressure compared to the control group(p＜0.05). The contraction intervals were increased after intravesical administration of methoctramine and tolterodine compared to the BOO group(p＜0.05). However, the contraction pressures were similar among the methoctramine, tolterodine and BOO groups. For the muscle strip study, the BOO group demonstrated increased contractile responses compared to the control group(p＜0.05). However, the contractile responses were decreased after administration of methoctramine(only in 32Hz) and tolterodine(p＜0.05).
Conclusions:The results of this study showed that detrusor overactivity induced by BOO in rats is primarily mediated by M3 muscarinic receptors in the detrusor muscle, and by M2 and M3 muscarinic receptors in the urothelium. (Korean J Urol 2007;48:1044-1049)
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KEYWORD
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Muscarinic receptor, Overactive detrusor, Urothelium, Bladder outlet obstruction
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